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Assessing the Biosynthetic Capabilities of Secretory Glands in Citrus Peel1[W][OA]

Identifieur interne : 001400 ( Main/Exploration ); précédent : 001399; suivant : 001401

Assessing the Biosynthetic Capabilities of Secretory Glands in Citrus Peel1[W][OA]

Auteurs : Siau Sie Voo ; Howard D. Grimes ; B. Markus Lange

Source :

Plant Physiology [ 0032-0889 ] ; 2012.

RBID : PMC:3375987

Abstract

Epithelial cells (ECs) lining the secretory cavities of Citrus peel have been hypothesized to be responsible for the synthesis of essential oil, but direct evidence for such a role is currently sparse. We used laser-capture microdissection and pressure catapulting to isolate ECs and parenchyma cells (as controls not synthesizing oil) from the peel of young grapefruit (Citrus × paradisi ‘Duncan’), isolated RNA, and evaluated transcript patterns based on oligonucleotide microarrays. A Gene Ontology analysis of these data sets indicated an enrichment of genes involved in the biosynthesis of volatile terpenoids and nonvolatile phenylpropanoids in ECs (when compared with parenchyma cells), thus indicating a significant metabolic specialization in this cell type. The gene expression patterns in ECs were consistent with the accumulation of the major essential oil constituents (monoterpenes, prenylated coumarins, and polymethoxylated flavonoids). Morphometric analyses demonstrated that secretory cavities are formed early during fruit development, whereas the expansion of cavities, and thus oil accumulation, correlates with later stages of fruit expansion. Our studies have laid the methodological and experimental groundwork for a vastly improved knowledge of the as yet poorly understood processes controlling essential oil biosynthesis in Citrus peel.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375987

DOI: 10.1104/pp.112.194233
PubMed: 22452856
PubMed Central: 3375987

Affiliations:

Le document en format XML

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<series><title level="j">Plant Physiology</title>
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<front><div type="abstract" xml:lang="en"><p>Epithelial cells (<xref ref-type="def" rid="def1">ECs</xref>
) lining the secretory cavities of <italic>Citrus</italic>
 peel have been hypothesized to be responsible for the synthesis of essential oil, but direct evidence for such a role is currently sparse. We used laser-capture microdissection and pressure catapulting to isolate <xref ref-type="def" rid="def1">ECs</xref>
 and parenchyma cells (as controls not synthesizing oil) from the peel of young grapefruit (<italic>Citrus</italic>
 × <italic>paradisi</italic>
 ‘Duncan’), isolated RNA, and evaluated transcript patterns based on oligonucleotide microarrays. A Gene Ontology analysis of these data sets indicated an enrichment of genes involved in the biosynthesis of volatile terpenoids and nonvolatile phenylpropanoids in <xref ref-type="def" rid="def1">ECs</xref>
 (when compared with parenchyma cells), thus indicating a significant metabolic specialization in this cell type. The gene expression patterns in <xref ref-type="def" rid="def1">ECs</xref>
 were consistent with the accumulation of the major essential oil constituents (monoterpenes, prenylated coumarins, and polymethoxylated flavonoids). Morphometric analyses demonstrated that secretory cavities are formed early during fruit development, whereas the expansion of cavities, and thus oil accumulation, correlates with later stages of fruit expansion. Our studies have laid the methodological and experimental groundwork for a vastly improved knowledge of the as yet poorly understood processes controlling essential oil biosynthesis in <italic>Citrus</italic>
 peel.</p>
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Assessing the Biosynthetic Capabilities of Secretory Glands in Citrus Peel1[W][OA]

Assessing the Biosynthetic Capabilities of Secretory Glands in Citrus Peel1[W][OA]

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Abstract

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